Leveraging the Grid to Provide a Global Platform for Ubiquitous Computing Research

The requirement for distributed systems support for Ubicomp has led to the development of numerous platforms, each addressing a subset of the overall requirements of ubiquitous systems. In contrast, many other scientific disciplines have embraced the vision of a global distributed computing platform, i.e. the Grid. We believe that the Grid has the potential to evolve into an ideal platform for building ubiquitous computing applications. In this paper we explore in detail the areas of synergy between Grid computing and ubiquitous computing and highlight a series of research challenges in this space

Network layer access control for context-aware IPv6 applications

As part of the Lancaster GUIDE II project, we have developed a novel wireless access point protocol designed to support the development of next generation mobile context-aware applications in our local environs. Once deployed, this architecture will allow ordinary citizens secure, accountable and convenient access to a set of tailored applications including location, multimedia and context based services, and the public Internet. Our architecture utilises packet marking and network level packet filtering techniques within a modified Mobile IPv6 protocol stack to perform access control over a range of wireless network technologies. In this paper, we describe the rationale for, and components of, our architecture and contrast our approach with other state-of-the- art systems. The paper also contains details of our current implementation work, including preliminary performance measurements

Genetic dissection of grain iron concentration in hexaploid wheat (Triticum aestivum L.) using a genome-wide association analysis method

Iron (Fe) is an essential micronutrient of the body. Low concentrations of bioavailable Fe in staple food result in micronutrient malnutrition. Wheat (Triticum aestivum L.) is the most important global food crop and thus has become an important source of iron for people. Breeding nutritious wheat with high grain-Fe content has become an effective means of alleviating malnutrition. Understanding the genetic basis of micronutrient concentration in wheat grains may provide useful information for breeding for high Fe varieties through marker-assisted selection (MAS). Hence, in the present study, genome-wide association studies (GWAS) were conducted for grain Fe. An association panel of 207 accessions was genotyped using a 660K SNP array and phenotyped for grain Fe content at three locations. The genotypic and phenotypic data obtained thus were used for GWAS. A total of 911 SNPs were significantly associated with grain Fe concentrations. These SNPs were distributed on all 21 wheat chromosomes, and each SNP explained 5.79–25.31% of the phenotypic variations. Notably, the two significant SNPs (AX-108912427 and AX-94729264) not only have a more significant effect on grain Fe concentration but also have the reliability under the different environments. Furthermore, candidate genes potentially associated with grain Fe concentration were predicted, and 10 candidate genes were identified. These candidate genes were related to transport, translocation, remobilization, and accumulationof ironin wheat plants. These findings will not only help in better understanding the molecular basis of Fe accumulation in grains, but also provide elite wheat germplasms to develop Fe-rich wheat varieties through breeding

The Long Noncoding RNA MALAT1 Induces Tolerogenic Dendritic Cells and Regulatory T Cells via miR155/Dendritic Cell-Specific Intercellular Adhesion Molecule-3 Grabbing Nonintegrin/IL10 Axis

By shaping T cell immunity, tolerogenic dendritic cells (tDCs) play critical roles in the induction of immune tolerance after transplantation. However, the role of long noncoding RNAs (lncRNAs) in the function and immune tolerance of dendritic cells (DCs) is largely unknown. Here, we found that the lncRNA MALAT1 is upregulated in the infiltrating cells of tolerized mice with cardiac allografts and activated DCs. Functionally, MALAT1 overexpression favored a switch in DCs toward a tolerant phenotype. Mechanistically, ectopic MALAT1 promoted dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) expression by functioning as an miR155 sponge, which is essential for the tolerogenic maintenance of DCs and the DC-SIGN-positive subset with more potent tolerogenic ability. The adoptive transfer of MALAT1-overexpressing DCs promoted cardiac allograft survival and protected from the development of experimental autoimmune myocarditis, accompanied with increasing antigen-specific regulatory T cells. Therefore, overexpressed MALAT1 induces tDCs and immune tolerance in heart transplantation and autoimmune disease by the miRNA-155/DC-SIGH/IL10 axis. This study highlights that the lncRNA MALAT1 is a novel tolerance regulator in immunity that has important implications in settings in which tDCs are preferred

Administration of Interleukin-35-Conditioned Autologous Tolerogenic Dendritic Cells Prolong Allograft Survival After Heart Transplantation

Background/Aims: IL-35, a powerful suppressor of inflammation and autoimmunity, is primarily secreted by regulatory T cells (Tregs) and can, in turn, promote Treg differentiation. However, the precise effect of IL-35 on dendritic cells (DCs) remains to be clarified. Methods: In this study, we investigated the expression of IL-35 in DCs after stimulation with LPS utilizing enzyme linked immunosorbent assay(ELISA), quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) and western blotting, and the influence of IL-35 on the maturation and function of DCs by mixed lymphocyte reaction assay and flow cytometry. We further examined the regulation of IL-35 in DCs by the microRNA let-7i (let-7i) via transfected with let-7i mimic, inhibitor or suppressor of cytokine signalling 1 (SOCS1) siRNA. IL-35-overexpressing DCs were transfused into BALB/c recipients with C57BL/6 heart transplantations to verify the role of immune tolerance in transplantation. Results: The results showed that IL-35 expression was significantly up-regulated following lipopolysaccharide (LPS)-induced DC maturation. Overexpression of IL-35 suppressed DC maturation, promoted the secretion of anti-inflammatory cytokines, and subsequently affected the balance between Treg and Th17 cells. IL-35 expression in DCs was regulated by let-7i, which targets SOCS1. The transfusion of IL-35-transfected DCs induced Treg generation in mice and prolonged cardiac allograft survival. Conclusion: Our data demonstrated that IL-35 induces tolerogenic DCs which are capable of alleviating allograft rejection. Clinical application of IL-35-treated DCs might be a promising approach for eliciting cardiac allograft immune tolerance

BnMs3 is required for tapetal differentiation and degradation, microspore separation, and pollen-wall biosynthesis in Brassica napus

7365AB, a recessive genetic male sterility system, is controlled by BnMs3 in Brassica napus, which encodes a Tic40 protein required for tapetum development. However, the role of BnMs3 in rapeseed anther development is still largely unclear. In this research, cytological analysis revealed that anther development of a Bnms3 mutant has defects in the transition of the tapetum to the secretory type, callose degradation, and pollen-wall formation. A total of 76 down-regulated unigenes in the Bnms3 mutant, several of which are associated with tapetum development, callose degeneration, and pollen development, were isolated by suppression subtractive hybridization combined with a macroarray analysis. Reverse genetics was applied by means of Arabidopsis insertional mutant lines to characterize the function of these unigenes and revealed that MSR02 is only required for transport of sporopollenin precursors through the plasma membrane of the tapetum. The real-time PCR data have further verified that BnMs3 plays a primary role in tapetal differentiation by affecting the expression of a few key transcription factors, participates in tapetal degradation by modulating the expression of cysteine protease genes, and influences microspore separation by manipulating the expression of BnA6 and BnMSR66 related to callose degradation and of BnQRT1 and BnQRT3 required for the primary cell-wall degradation of the pollen mother cell. Moreover, BnMs3 takes part in pollen-wall formation by affecting the expression of a series of genes involved in biosynthesis and transport of sporopollenin precursors. All of the above results suggest that BnMs3 participates in tapetum development, microspore release, and pollen-wall formation in B. napus

Slow slip source characterized by lithological and geometric heterogeneity

Slow slip events (SSEs) accommodate a significant proportion of tectonic plate motion at subduction zones, yet little is known about the faults that actually host them. The shallow depth (<2 km) of well-documented SSEs at the Hikurangi subduction zone offshore New Zealand offers a unique opportunity to link geophysical imaging of the subduction zone with direct access to incoming material that represents the megathrust fault rocks hosting slow slip. Two recent International Ocean Discovery Program Expeditions sampled this incoming material before it is entrained immediately down-dip along the shallow plate interface. Drilling results, tied to regional seismic reflection images, reveal heterogeneous lithologies with highly variable physical properties entering the SSE source region. These observations suggest that SSEs and associated slow earthquake phenomena are promoted by lithological, mechanical, and frictional heterogeneity within the fault zone, enhanced by geometric complexity associated with subduction of rough crust

Adaptive privacy management for distributed applications

In networked computing environments, it becomes increasingly difficult for normal people to manage privacy, i.e., “to determine for themselves when, how, and to what extent information about them is communicated with others”. The thesis argues that achieving better privacy is not about hiding as much personal information as possible but enabling personal information disclosure at a level of openness that is as close as to a user’s desired level to assist him/her in accomplishing useful tasks. Following Palen and Dourish’s observation that privacy management is a dialectic and dynamic boundary regulation process [Palen03], the thesis argues that no set of pre-specified static privacy policies can meet users' changing requirements for privacy in networked computing environments, and therefore a new approach (i.e., adaptive privacy management) is proposed as the process that a user and/or a system to continuously adjust the system behaviour of disclosing personal information according to the user's changing desire for openness. In this thesis, we propose a set of requirements for adaptive privacy management and i the design and implementation of a middleware that meets these requirements for the target domain of applications that enable intentional sharing of personal information in networked computing environments. The middleware facilitates the creation of adaptive privacy aware applications that allows users or the system on behalf of the user to adjust the balance between openness and closedness; leading to an evolution of the users’ privacy preferences as a result of on-going interactions. A prototype adaptive privacy management system was implemented based on this middleware; demonstrating the feasibility of adaptive privacy management for the target domain. Both the principles of adaptive privacy management and the prototype implementation were evaluated based on the results of a detailed user study using a GSM location sharing application constructed using the prototype platform. The study reveals the our core requirements are important for end users, and that our supporting design did provide adequate support for the characteristics we propose

Mobile context-aware game for the next generation

Traditional networked multiplayer games are typically confined to be played on desktop computers. With the recent advances in mobile networking, context-aware computing, and sensor-based computing, researchers and game designers are able to explore the potential of combining these new technologies together to develop mobile networked, context-aware, augmented reality multiplayer games. As part of the new research collaboration between Lancaster University, Cisco Systems, Microsoft Research and Orange – MIPv6 Systems Research Lab (MSRL), such a mobile context-aware multiplayer game is proposed and explored. The proposed game, named Real Tournament, gathers real-time contextual information, e.g., physical location, orientation and other personal facets from the players and injects them into the game arena to generate game events. It is not only the interaction with the virtual game world that drives the game process, but the interaction with the physical world as well. This paper introduces the game scenario that enabled us to explore the features in the aforementioned areas simultaneously. The proposed game architecture and components are described in detail, and the benefits of our architecture are showed by comparison with the existing approaches. The paper also describes the wireless overlay network infrastructure, and demonstrates how the advanced features in Mobile IPv6 are explored during the development of the game. Moreover, a security mechanism is designed at the network-level, and it can be easily utilized for other network services

Real tournament - mobile context aware gaming for the next generation

With the recent advances in mobile networking, context-aware computing, and sensor-based computing, researchers and game designers are able to explore the potential of combining these new technologies to develop mobile, networked, context-aware, augmented reality multiplayer games. As part of new research collaboration between Lancaster University, Cisco Systems, Microsoft Research and Orange – MIPv6 Systems Research Lab, such a mobile context-aware multiplayer game is proposed and explored. The proposed game, Real Tournament, gathers real-time contextual information, e.g. physical location and orientation, from the players and injects them into the game engine to generate game events